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KMID : 1140120100150010039
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Cancer Prevention Research
2010 Volume.15 No. 1 p.39 ~ p.44
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The Pro-apoptotic Effects of Thalidomide on a Pancreatic Cancer Cell Line
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Lim Seong-Uk
Kim Dae-Eun
Cho Dae-Ho
Yoon Ju-Young
Lee Ji-Hee
Hwang Jun-Eul
Shim Hyun-Jeong
Bae Woo-Kyun
Cho Sang-Hee
Chung Ik-Joo
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Abstract
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Thalidomide has been shown to have therapeutic potential in a number of conditions including cancer. The aim of this study was to evaluate the apoptotic effects of thalidomide on a pancreatic cancer cell line. Treatment with thalidomide decreased the viability of BxPc-3 cells in dose dependent manner. Using flow cytometry analysis, determination of whether thalidomide treatment of BxPc-3 cells at concentrations of 10, 25, and 50 μg/ml affected the number of annexin V-positive cells was evaluated. The percent of apoptotic cells were 9.5±2.6%, 21.7±3.6% and 29.3±4.7% respectively in the BxPc-3 cells at 10 μg/ml, 25 μ/ml and 50 μg/ml of thalidomide treated for 24 hours. Thalidomide induced the expression of pro-apoptotic Bax in the BxPc-3 cells and increased the Bax: Bcl-2 ratio. In addition, the results of this study showed that thalidomide increased apoptosis induced by gemcitabine.
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KEYWORD
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Thalidomide, Apoptosis, Pancreatic cancer
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